FastQCFastQC Report
Fri 31 Oct 2014
MK199_GTGAAA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK199_GTGAAA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8474045
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG200230.23628621278267933TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT41900.032.75161412
GCACACG42650.032.2811711
CGTCTGA42600.031.68591716
CACGTGA37350.030.72060831
ACGTCTG44300.030.62196515
ACGTGAA37850.030.19537232
CGTGAAA37950.029.93795633
CACGTCT46200.029.55739814
CTGAACT49400.027.7348119
AGCACAC50200.027.6053910
GTCACGT44200.026.67316829
GTCTGAA51150.026.60935417
TCACGTG43950.026.36369530
TCTGAAC52100.026.12446218
CTCGTAT45500.025.43039944
CAGTCAC49500.025.27233927
GTGAAAC45600.024.07616234
ACACGTC58750.023.32001913
TGAAACG47500.023.25712635
TCGTATG50050.022.75872245
Produced by FastQC (version 0.11.2)