FastQCFastQC Report
Fri 31 Oct 2014
MK198_ACTTGA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK198_ACTTGA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3801150
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC56750.1492969232995278TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC14500.034.13636829
GCACACG17850.032.0153411
CACACGT18300.030.98218312
CGTCTGA19700.029.46526316
CACGTCT19550.029.46117414
ACGTCTG20100.028.99082615
GTATGCC16100.027.81659945
AGCACAC20700.027.28135710
GTCTGAA22550.026.14036417
AGTCACA18750.025.43950328
CGTATGC17600.025.31799944
ACACTTG19250.025.24559432
ACACGTC22600.025.08701313
CTCGTAT18500.024.81587442
CTGAACT23650.024.35406119
CAGTCAC20800.024.33846527
TCACACT20900.024.11372630
TCTGAAC24100.024.08571418
CACACTT21850.023.27124231
CCAGTCA22700.022.7966126
Produced by FastQC (version 0.11.2)