FastQCFastQC Report
Fri 31 Oct 2014
MK197_GTCCGC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK197_GTCCGC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5698163
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG420390.7377640829158449TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACA53550.041.9713536
CGCACAT57100.039.64156337
CACACGT61850.039.06610512
GCACACG62150.038.9499311
ACGTCTG63000.038.3172815
CACGTCT63350.037.92801714
CGTCTGA64400.037.589116
TCACGTC62800.036.75654230
GTCACGT63350.036.72250729
CTCGTAT62750.036.62809844
TCCGCAC61300.036.59161835
AGCACAC67100.036.3112710
GTCTGAA67300.036.23679417
GCACATC63500.035.93123638
CTGAACT66900.035.88208419
CGTCCGC63500.035.6769333
TCGTATG64350.035.6474145
CACATCT63800.035.5172739
CAGTCAC68250.035.10855527
AGTCACG68300.034.9178428
Produced by FastQC (version 0.11.2)