FastQCFastQC Report
Fri 31 Oct 2014
MK196_ACAGTG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK196_ACAGTG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493607
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC71731.453180364135841TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC11550.043.6308629
CACACGT13000.042.40279812
TCACACA11850.042.33643330
CACACAG11950.041.98215531
ACACAGT12100.041.83356532
CACGTCT13400.041.80866614
GCACACG13250.041.7683211
AGTCACA12150.041.66563428
CAGTCAC12100.041.6518627
CACAGTG12550.041.4091233
GTATGCC12200.041.327245
CTCGTAT12450.041.220542
CGTATGC12350.041.00750444
ACGTCTG13800.040.7598615
CTGAACT13600.040.52797719
CCAGTCA12650.040.37449326
AGCACAC13450.040.310910
TCTGAAC13650.040.21878418
CGTCTGA13950.039.99901216
GTCTGAA13850.039.80045717
Produced by FastQC (version 0.11.2)