MK195_CGATGT_L005_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	MK195_CGATGT_L005_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7959148
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC	408394	5.131127100538902	TruSeq Adapter, Index 2 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTCTGCC	20937	0.263055794414176	TruSeq Adapter, Index 2 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGTATCTCGTATGCC	16594	0.20848965241003184	TruSeq Adapter, Index 2 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTCTCTCGTATGCC	14305	0.17973029273987617	TruSeq Adapter, Index 2 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGAC	10054	0.12632005335244426	TruSeq Adapter, Index 2 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACCGATGTATCTCGTATGCC	9269	0.11645718863375829	TruSeq Adapter, Index 2 (98% over 51bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACCGATGTATCTCGTATGCC	8496	0.10674509382160001	TruSeq Adapter, Index 2 (98% over 51bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CACACGT	73555	43.608883	12
GTATGCC	63330	43.53553	45
ACACGTC	73855	43.504856	13
CACGTCT	74545	43.47861	14
ACGTCTG	75100	43.408947	15
CTCGTAT	65375	43.326916	42
GCACACG	74130	43.29794	11
GAACTCC	69840	43.29395	21
AGTCACC	67865	43.2493	28
TCGTATG	67015	43.237144	43
CTCCAGT	69965	43.1925	24
TCTCGTA	65765	43.189754	41
AGCACAC	73370	43.16074	10
CGTATGC	64630	43.1543	44
CCGATGT	66995	43.149967	33
CGTCTGA	74195	43.116673	16
ACTCCAG	69630	43.106255	23
CAGTCAC	68635	43.049297	27
TCCAGTC	69420	43.03894	25
TGTATCT	68110	43.028137	37