MK194_ATCACG_L005_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	MK194_ATCACG_L005_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12280275
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC	489040	3.9823212428060444	TruSeq Adapter, Index 1 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATGCC	22778	0.18548444558448407	TruSeq Adapter, Index 1 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTCTGCC	22226	0.1809894322399132	TruSeq Adapter, Index 1 (98% over 51bp)
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGT	20593	0.16769168442889107	No Hit
GGCATAAGCTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGA	19579	0.15943454035027718	No Hit
CTCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG	19211	0.15643786478722993	No Hit
GCTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACGA	14404	0.11729379024492528	No Hit
GCGCCTGCTGCCTTCCTTGGACGTGGTAGCCGTTTCTAAGGCTCCCTCTCC	13369	0.10886564022385492	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ACGTCTG	85560	43.858543	15
GCACACG	85150	43.736797	11
CACACGT	85020	43.716526	12
GTCTGAA	83525	43.647808	17
GTATGCC	74055	43.641	45
CGTCTGA	84405	43.54175	16
CACGTCT	85890	43.519764	14
GTCACAT	81445	43.21573	29
CTGAACT	83675	43.16104	19
TCTGAAC	83755	43.111755	18
ACACGTC	86370	43.033222	13
ACATCAC	77815	42.981735	32
CTCGTAT	77455	42.73358	42
CGTATGC	76845	42.700855	44
TCACATC	80425	42.46544	30
TCGTATG	79815	42.431553	43
CACATCA	79590	42.339943	31
GAGCACA	87950	42.247177	9
GAACTCC	82785	42.2098	21
TCTCGTA	78500	42.193375	41