FastQCFastQC Report
Fri 31 Oct 2014
MK193_GGTAGC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK193_GGTAGC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16842383
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC245518714.577432421528474TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGTACTCCAGTCACGGTAGCATCTCGTATGCC204100.12118237662687044TruSeq Adapter, Index 11 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2835450.044.2585812
ACGTCTG2837950.044.1964715
GCACACG2846050.044.1451211
GTCTGAA2827600.044.01956617
CACGTCT2851350.044.01717414
CGTCTGA2824250.044.0006216
AGCACAC2861500.043.96966610
GTATGCC2770200.043.89215545
CTGAACT2839300.043.7797819
GGTAGCA2754650.043.7786434
TCTGAAC2843100.043.7384218
GTAGCAT2757200.043.73142235
AGAGCAC2892550.043.691348
TAGCATC2752850.043.67780336
GATCGGA2830450.043.675351
ACACGTC2876500.043.66985713
CAGTCAC2848300.043.6649727
CGGTAGC2765950.043.65493433
ACGGTAG2801000.043.6283432
AGTCACG2852800.043.5688928
Produced by FastQC (version 0.11.2)