FastQCFastQC Report
Fri 31 Oct 2014
MK189_GTGGCC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK189_GTGGCC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29217931
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC2048920.701254308527185TruSeq Adapter, Index 20 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC253750.039.54742445
CGTGGCC251900.038.69016633
CGTATGC265850.037.82365444
GTGGCCA266700.036.54282434
ACGTCTG290400.036.20171415
CACACGT296800.035.4210212
TCGTATG286500.035.20738643
CGTCTGA299000.035.1830916
CTCGTAT290550.034.7631142
GTCTGAA306200.034.40728817
CAGTCAC301250.033.8932727
TCACGTG296750.033.60865430
CTGAACT316450.033.30026219
GTCACGT305350.032.91429
GCACACG321300.032.8531111
TCTGAAC327400.032.2894918
CACGTCT326850.032.19206214
TGGCCAT304400.032.05514535
TCTCGTA317850.031.82319341
GGCCATC314750.031.2081536
Produced by FastQC (version 0.11.2)