FastQCFastQC Report
Fri 31 Oct 2014
MK188_GTGAAA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK188_GTGAAA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15496243
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG1845341.1908305774502892TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG361650.041.27147715
CGTGAAA313000.040.5904533
ACGTGAA321150.040.4500732
CGTCTGA363100.040.4126716
CACACGT365300.040.39782712
GTCTGAA362500.040.2439717
CACGTGA331050.040.18519631
TCGTATG325900.040.1506445
CAGTCAC339300.040.0447527
GTCACGT346250.039.86386529
GCACACG371850.039.77583311
CACGTCT374000.039.6619914
TCACGTG345350.039.6547930
GTGAAAC310000.039.4664434
CTGAACT368100.038.9888119
CTCGTAT329350.038.9715344
AGTCACG352900.038.7310328
TGAAACG315100.038.64201435
TCTGAAC373100.038.4915518
AGCACAC383800.038.3497910
Produced by FastQC (version 0.11.2)