FastQCFastQC Report
Fri 31 Oct 2014
MK187_GTCCGC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK187_GTCCGC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15125635
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG1968671.3015453566081687TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACA322550.041.85835636
CACACGT412800.041.4939412
CGCACAT336050.041.45515437
ACGTCTG421400.041.3992615
GTCTGAA417000.041.08117
CGTCTGA421400.041.0148516
TCGTATG376900.040.81884445
CGTCCGC365500.040.58366833
GCACACG423300.040.55449711
CAGTCAC391050.040.31475427
GTCACGT399800.040.30443629
CTGAACT420350.040.10542719
CACGTCT430500.040.0328914
TCCGCAC351400.039.98435
TCACGTC389800.039.9295430
TCTGAAC421600.039.89098718
GTCCGCA361150.039.8639334
CTCGTAT381200.039.6735644
ACACGTC435500.039.3672713
AGCACAC431700.039.34845410
Produced by FastQC (version 0.11.2)