FastQCFastQC Report
Fri 31 Oct 2014
MK186_GTAGAG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK186_GTAGAG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15834492
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC608330.38418030714215523TruSeq Adapter, Index 3 (97% over 37bp)
CTCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG177620.11217284394093602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGTA115150.036.96670530
GTATGCC113400.036.8158945
ACGTCTG139350.036.0692115
CACACGT137800.035.9363512
CACGTAG122150.035.10613331
CGTATGC122600.034.64063644
GCACACG147550.033.6830511
CGTCTGA146900.033.67936316
GTCTGAA145800.033.45508617
CAGTCAC134750.032.79270627
TCGTATG134250.032.70750443
CACGTCT154750.032.3489214
ACGTAGA129250.031.97649232
GTCACGT141050.031.58253929
AGCACAC157050.031.3590410
CTGAACT155200.031.28345319
ACACGTC159600.031.2392113
CTCGTAT138900.030.85099242
TCTGAAC157600.030.69323718
AGTCACG145100.030.59315328
Produced by FastQC (version 0.11.2)