FastQCFastQC Report
Fri 31 Oct 2014
MK184_ATGTCA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK184_ATGTCA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17740296
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG2882321.6247305005508366TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG571450.041.67893215
CACATGT515750.041.46350531
CGTCTGA566350.041.33520516
CACACGT567350.041.33011212
TCACATG523700.041.2722730
AGTCACA522800.041.25073228
GTCTGAA565050.041.2155217
GTCACAT527400.041.1538229
ACATGTC511000.041.1400732
ATGTCAG511650.041.10099834
TCGTATG511950.040.87795645
GCACACG573950.040.86165211
CTGAACT565450.040.68432219
CATGTCA516300.040.50858733
CAGTCAC537550.040.4829927
CACGTCT585700.040.3422114
TGTCAGA505150.040.24472835
TCTGAAC572000.040.22317518
CTCGTAT510900.040.08096744
ACACGTC601950.039.10023513
Produced by FastQC (version 0.11.2)