FastQCFastQC Report
Fri 31 Oct 2014
MK183_AGTTCC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK183_AGTTCC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5747887
Sequences flagged as poor quality0
Sequence length51
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG3903046.790390973239384TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTCTG193360.33640188124783943TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTCTCTCGTATG152440.26521050257251055TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTTCCGTATCTCGTATG95860.1667743294187934TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCAGTTCCGTATCTCGTATG93500.16266847277964927TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACAGTTCCGTATCTCGTATG89400.1555354167540176TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCAGTATCTCGTATG77690.1351627128369086TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTACGTATCTCGTATG76790.13359692005079432TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATATCGTATG63270.11007523286383326TruSeq Adapter, Index 14 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT686000.044.231512
GTCTGAA674550.044.07838417
CACGTCT691400.044.06827514
CGTCTGA682550.044.0562116
TCGGAAG704550.044.0422173
ACGTCTG696950.044.020815
ACACGTC690600.043.99878313
ATCGGAA705950.043.9333272
AGCACAC680300.043.93173210
AGAGCAC683350.043.926618
GAGCACA683900.043.907739
AAGAGCA684550.043.862757
TCTGAAC671250.043.81241218
GATCGGA714700.043.7651941
AGTCACA624650.043.75914428
GCACACG695050.043.69537411
CTGAACT673250.043.6903819
GGAAGAG700250.043.6793375
CGGAAGA697400.043.677564
CACAGTT622650.043.6380231
Produced by FastQC (version 0.11.2)