FastQCFastQC Report
Fri 31 Oct 2014
MK182_AGTCAA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK182_AGTCAA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10696118
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC137807212.883851879719352TruSeq Adapter, Index 13 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACATCTCGTATGCC606970.5674675615957117TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTCTGCC581150.5433279625374365TruSeq Adapter, Index 13 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACTCTCGTATGCC545260.5097737328627078TruSeq Adapter, Index 13 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCAAATCTCGTATGCC378720.35407238401820174TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCCAATCTCGTATGCC352940.32997018170517567TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGAC305710.2858139747523354TruSeq Adapter, Index 13 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCAGTCAAATCTCGTATGCC252840.23638482671937613TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACAGTCAAATCTCGTATGCC244400.22849411347182222TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATATCGTATGCC228660.21377849421631284TruSeq Adapter, Index 13 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCA227700.21288097233033518TruSeq Adapter, Index 13 (98% over 50bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACAGTCAAATCTCGTATGCC214970.20097945815481844TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGCGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC175950.1644989331643499TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTAAAATCTCGTATGCC171760.16058162409950974TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTAGTATGCC161010.150531248813822TruSeq Adapter, Index 13 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTACAGTCACAGTCAAATCTCGTATGCC145370.13590912142143532TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGACCTCCAGTCACAGTCAAATCTCGTATGCC145360.13589977223512306TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCAAGTCACAGTCAAATCTCGTATGCC108850.10176589300903374TruSeq Adapter, Index 13 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2566500.044.6085241
ATCGGAA2543400.044.587852
TCGGAAG2557000.044.4312823
CACACGT2494300.044.4213812
CGGAAGA2510800.044.378774
GAGCACA2481600.044.348079
AGAGCAC2485750.044.3075148
ACACGTC2503050.044.25800313
AAGAGCA2481650.044.2528847
AGCACAC2467700.044.24776510
CACGTCT2518900.044.23215514
ACGTCTG2531100.044.22695515
GGAAGAG2529450.044.226785
GCACACG2511150.044.18995711
GAAGAGC2504950.044.1689646
GTCTGAA2480350.044.09911717
CGTCTGA2505100.044.09052316
TCTGAAC2460600.044.0059518
CTGAACT2466550.043.88620819
GAACTCC2397300.043.73888421
Produced by FastQC (version 0.11.2)