FastQCFastQC Report
Fri 31 Oct 2014
MK181_CTTGTA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK181_CTTGTA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8443371
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC5184326.140106836475621TruSeq Adapter, Index 12 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTCTGCC222480.2634966531732409TruSeq Adapter, Index 12 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTACTCTCGTATGCC215980.25579830615047TruSeq Adapter, Index 12 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCATCTCGTATGCC185740.219983227078379TruSeq Adapter, Index 12 (98% over 51bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACCTTGTAATCTCGTATGCC138130.16359579603928337TruSeq Adapter, Index 12 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGAC104460.1237183584613302TruSeq Adapter, Index 12 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACCTTGTAATCTCGTATGCC101500.12021264966326838TruSeq Adapter, Index 12 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCCTTGTAATCTCGTATGCC95530.11314201401312343TruSeq Adapter, Index 12 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT873500.044.19484312
GCACACG876900.044.12198311
GTATGCC773850.044.118745
ACGTCTG890650.044.0986415
CACGTCT885100.043.97099314
AGTCACC804850.043.88718428
GTCACCT821600.043.86371629
CGTCTGA876850.043.83816516
ACACGTC882600.043.8054513
AGCACAC872250.043.76910410
GTCTGAA874450.043.70401817
GATCGGA921450.043.548751
TCTGAAC869600.043.50016818
CTGAACT870650.043.4676119
CTCGTAT808950.043.3784542
ACCTTGT838050.043.3590132
GTAATCT790400.043.31431637
CGTATGC797550.043.2873844
TGTAATC791000.043.28435536
AGAGCAC888200.043.2642828
Produced by FastQC (version 0.11.2)