FastQCFastQC Report
Fri 31 Oct 2014
MK180_GGCTAC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK180_GGCTAC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9197035
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC200530.2180376610505451TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA42350.037.1347332
GCTACAT42500.035.7331635
GGCTACA42150.035.49609834
CACGGCT45650.035.48528331
CGGCTAC45450.034.3543833
GTATGCC45050.034.21998645
CTACATC42850.034.0761436
CACACGT59900.033.6921912
ACGTCTG61900.033.22113415
CGTCTGA62050.032.9232716
GCACACG62000.032.80484811
TCACGGC50100.032.51302330
ACACGTC63000.031.92718313
CACGTCT66700.030.66175314
GTCTGAA66900.030.3684717
TACATCT50200.029.76322437
CTGAACT69000.028.85728519
AGCACAC69600.028.8024710
CAGTCAC59750.028.46784427
TCTGAAC71050.028.21466418
Produced by FastQC (version 0.11.2)