FastQCFastQC Report
Fri 31 Oct 2014
MK178_GATCAG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK178_GATCAG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14261651
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC143816910.084169076918235TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1653800.044.17975212
GTATGCC1630450.044.090445
ACGTCTG1656400.044.05879615
GCACACG1662350.044.01748711
CACGTCT1658950.043.98835814
CGTCTGA1661650.043.8764216
ACACGTC1665700.043.8452213
AGCACAC1673700.043.7712510
GATCGGA1650400.043.6711041
GTCTGAA1673500.043.6695517
CAGATCT1603650.043.4961737
CGTATGC1654750.043.45789744
CAGTCAC1666800.043.44893627
TCTGAAC1678850.043.42869618
AGTCACG1666600.043.3986528
GTCACGA1645500.043.3895129
CTGAACT1680900.043.38020319
CTCGTAT1655000.043.332742
AGAGCAC1699950.043.3282748
TCGGAAG1663250.043.3277553
Produced by FastQC (version 0.11.2)