FastQCFastQC Report
Fri 31 Oct 2014
MK177_ACTTGA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK177_ACTTGA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19549332
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC973710.49807839981437735TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC131350.037.632129
GTATGCC132650.037.55580545
CACACGT143550.036.60976812
GCACACG151050.034.98562611
CGTCTGA153250.034.07234616
ACGTCTG154250.033.90979415
ACACTTG149850.032.54883632
ACACGTC162050.032.48584713
CACGTCT163100.032.09739714
GTCTGAA164200.031.97836117
AGTCACA158700.031.2887628
AGCACAC171600.030.84835410
CTGAACT171500.030.73571419
TCGTATG163200.030.73250643
CTCGTAT164350.030.5311642
CAGTCAC167050.030.41191127
TCTGAAC175600.030.09487518
TCACACT166900.030.06009530
CACACTT167450.029.94754431
CGTATGC170800.029.45727544
Produced by FastQC (version 0.11.2)