FastQCFastQC Report
Fri 31 Oct 2014
MK175_GCCAAT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK175_GCCAAT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13695430
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC3349222.4455018936973865TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT412450.043.10175712
GCACACG418550.042.5165911
ACACGTC421850.042.05615613
CACGTCT421050.042.01317214
TATCTCG410250.041.85840639
ACGTCTG423800.041.7777115
AGCACAC427500.041.7106810
GTATGCC416100.041.62941445
CGTCTGA424600.041.6089216
CTCGTAT415800.041.43191542
AGTCACG421500.041.3363328
CGTATGC420550.041.18356344
TCGGAAG428500.041.0917633
GTCTGAA433350.040.98681617
TCGTATG423500.040.9656443
GATCGGA430200.040.9528471
AGAGCAC439450.040.8938378
CAGTCAC426050.040.8900527
TCTGAAC431400.040.8749818
TCTCGTA422800.040.8101141
Produced by FastQC (version 0.11.2)