FastQCFastQC Report
Fri 31 Oct 2014
MK174_ACAGTG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK174_ACAGTG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15728648
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC11488977.304486692053888TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1327500.044.15077212
GCACACG1332850.043.99718511
CGTCTGA1333600.043.77829716
ACACGTC1341350.043.68972413
ACGTCTG1339600.043.67626615
GTATGCC1320000.043.6383645
AGCACAC1347500.043.58228710
CACGTCT1343800.043.5246914
GTCACAC1313150.043.4972629
GTCTGAA1347000.043.47306417
CACACAG1312700.043.3598331
ACACAGT1307500.043.3583432
CACAGTG1308050.043.27461633
CTGAACT1348200.043.2563719
TCACACA1319800.043.23792630
TCTGAAC1351200.043.20334618
AGTCACA1331800.043.0662128
TCGGAAG1350700.042.9343533
CAGTCAC1341850.042.9155127
GATCGGA1356000.042.791571
Produced by FastQC (version 0.11.2)