MK173_TGACCA_L005_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	MK173_TGACCA_L005_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1964208
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC	71830	3.656944682029602	TruSeq Adapter, Index 4 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTCTGCC	3512	0.17879980124304554	TruSeq Adapter, Index 4 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCACTCTCGTATGCC	3386	0.17238500199571533	TruSeq Adapter, Index 4 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCCATCTCGTATGCC	2435	0.1239685410099134	TruSeq Adapter, Index 4 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCCAATCTCGTATGCC	2021	0.10289134348297126	TruSeq Adapter, Index 4 (98% over 51bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CACACGT	13835	44.13653	12
GATCGGA	14440	43.91	1
GCACACG	13935	43.89941	11
CACGTCT	14035	43.859142	14
ACACGTC	13920	43.81853	13
TCGGAAG	14385	43.807518	3
AGCACAC	13870	43.65095	10
CGTCTGA	13945	43.593655	16
ACGTCTG	14260	43.59311	15
ATCGGAA	14415	43.59149	2
AGAGCAC	14215	43.145496	8
GAGCACA	14175	43.124397	9
TCTGAAC	13780	42.989075	18
GTCTGAA	14050	42.97963	17
AGTCACT	13105	42.972576	28
GTATGCC	12405	42.964252	45
CTCGTAT	12650	42.95059	42
CGGAAGA	14575	42.81967	4
CAGTCAC	12955	42.705975	27
AACTCCA	12910	42.663128	22