FastQCFastQC Report
Fri 31 Oct 2014
MK172_TTAGGC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK172_TTAGGC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16835328
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC906920.5387005230904917TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG154800.043.12304732
CACTTAG153550.042.97616631
CTTAGGC156550.042.10923833
TCACTTA157400.041.9249730
TTAGGCA161650.039.9030234
GTATGCC167950.039.51524445
AGTCACT174650.039.12489728
ACGTCTG201950.038.9476615
CACACGT204000.037.89522612
CGTCTGA207200.037.47218316
CACGTCT210050.037.08157314
GCACACG208800.037.01329411
CGTATGC184850.036.7182344
AGGCATC173200.036.64462736
GTCTGAA210350.036.4725117
CTCGTAT187650.036.39723642
ACACGTC215150.035.92023513
GTCACTT196100.035.78517529
TCGTATG196650.035.5557243
TAGGCAT183350.035.37671735
Produced by FastQC (version 0.11.2)