MK171_CGATGT_L006_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	MK171_CGATGT_L006_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8848204
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC	303619	3.4314195287540836	TruSeq Adapter, Index 2 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTCTGCC	18283	0.20662950356931192	TruSeq Adapter, Index 2 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGTATCTCGTATGCC	14711	0.16625972909304532	TruSeq Adapter, Index 2 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTCTCTCGTATGCC	12918	0.14599572975487454	TruSeq Adapter, Index 2 (98% over 51bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CACACGT	55940	43.87185	12
GCACACG	56450	43.551212	11
ACACGTC	56365	43.548542	13
AGCACAC	55815	43.333202	10
TCGGAAG	57955	43.324554	3
GATCGGA	58495	43.317726	1
CACGTCT	57035	43.309155	14
CGTCTGA	56375	43.149704	16
AGTCACC	51565	43.14534	28
ACGTCTG	57615	43.119186	15
AGAGCAC	56510	43.05905	8
TATCTCG	50685	42.985554	39
ATCGGAA	58240	42.946426	2
GTCTGAA	56255	42.857807	17
CTCGTAT	49940	42.81767	42
GAGCACA	57060	42.71893	9
GTATGCC	48795	42.716995	45
GTATCTC	50915	42.688515	38
TGTATCT	51870	42.636196	37
ATGTATC	49065	42.626793	36