FastQCFastQC Report
Fri 31 Oct 2014
MK170_ATCACG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK170_ATCACG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11788673
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC5371174.556212560989689TruSeq Adapter, Index 1 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATGCC290150.2461260906974008TruSeq Adapter, Index 1 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTCTGCC282340.23950108718767582TruSeq Adapter, Index 1 (98% over 51bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACATCACGATCTCGTATGCC127830.10843459649784162TruSeq Adapter, Index 1 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACGATCTCGTATGCC126810.10756935916366497TruSeq Adapter, Index 1 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACATCACGATCTCGTATGCC121870.10337889599618211TruSeq Adapter, Index 1 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT950500.044.16451312
GCACACG958750.043.87130711
ACACGTC957350.043.840913
AGCACAC947300.043.7848110
TCGGAAG980900.043.755093
CACGTCT965500.043.75279214
ACGTCTG971350.043.6421615
GATCGGA990300.043.6020471
CGTCTGA954150.043.49983616
AGAGCAC964400.043.414398
ATCGGAA986550.043.3427662
GTCTGAA944950.043.3400217
GAGCACA965100.043.3152929
AGTCACA880100.043.2301628
TCTGAAC938250.043.2202818
GTCACAT905000.043.17137529
GTATGCC845450.043.15368345
CTCGTAT867250.042.99959642
CGGAAGA984700.042.9419064
CAGTCAC908800.042.93235827
Produced by FastQC (version 0.11.2)