FastQCFastQC Report
Fri 31 Oct 2014
LW276_TTAGGC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW276_TTAGGC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14118317
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC731780.5183195702433937TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAG83000.039.8199731
ACTTAGG85550.038.5015632
GCACACG99600.036.7299211
CGTATGC93750.036.53570644
GTATGCC94950.036.00285745
ACACGTC102150.035.96719413
ACGTCTG102150.035.9228915
CACACGT101850.035.89641612
AGGCATC92850.035.32936
CACGTCT104600.035.1460114
CTCGTAT97850.035.14282242
GGCATCT94900.034.70992337
CGTCTGA106900.034.36878616
TCGTATG100200.034.34107643
CAGTCAC102950.033.81040627
TTAGGCA97100.033.71315834
ACTCCAG107200.033.54029523
GAACTCC108950.033.497221
CCAGTCA105400.033.4300926
TCCAGTC109700.032.42725425
Produced by FastQC (version 0.11.2)