FastQCFastQC Report
Fri 31 Oct 2014
LW275_CGATGT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW275_CGATGT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39842908
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC3658980.9183516424052179TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG471600.040.08882511
GTCACCG450100.040.01484329
ACGTCTG476500.039.64760215
AGTCACC456650.039.4667628
CACACGT479750.039.41248712
GTATGCC459950.039.34304445
ACACGTC480150.039.34216713
CGTATGC460850.039.2076144
CACGTCT483450.039.0727914
CGATGTA459050.038.53244434
CTCGTAT469750.038.52705442
CGTCTGA491650.038.44875716
CCGATGT460350.038.4236333
CAGTCAC475850.038.2531227
TCGTATG480000.037.80748743
ACCGATG473300.037.77641732
CCAGTCA486700.037.71933426
GAACTCC496650.037.69758221
AGCACAC505800.037.49384710
GTATCTC475750.037.4532738
Produced by FastQC (version 0.11.2)