FastQCFastQC Report
Fri 31 Oct 2014
LW274_ATCACG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW274_ATCACG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7056189
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC98460213.95373621653275TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1108450.044.36670312
ACACGTC1107900.044.3481113
GCACACG1111500.044.26317211
ACGTCTG1108550.044.21016715
CACGTCT1109850.044.17865414
AGCACAC1116950.044.12978710
CGTCTGA1111800.044.0364116
AGAGCAC1124650.043.9753768
GAGCACA1124500.043.927229
GTATGCC1090150.043.8619645
GAACTCC1108950.043.8549321
CTCCAGT1105400.043.70673824
CTGAACT1117650.043.67677319
TCGGAAG1137100.043.6666533
CCAGTCA1105500.043.6580126
ACTCCAG1108550.043.6474923
TGAACTC1117800.043.64536320
TCCAGTC1106550.043.6389525
GATCGGA1124200.043.6338651
TCTGAAC1120400.043.63382718
Produced by FastQC (version 0.11.2)