FastQCFastQC Report
Fri 31 Oct 2014
LW273_GGTAGC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW273_GGTAGC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5231924
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC104484719.97060737120799TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGGAGCATCTCGTATGCC101000.19304561763511854TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGTACTCCAGTCACGGTAGCATCTCGTATGCC92650.17708590568211618TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGCATCTCGTATGCC56640.10825845329557539TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1191400.044.35698312
GCACACG1193550.044.33551811
ACACGTC1193000.044.28806313
AGCACAC1196950.044.2805910
ACGTCTG1193800.044.22215715
TCGGAAG1207650.044.2198833
GATCGGA1198500.044.206571
AGAGCAC1202200.044.2009548
CACGTCT1195150.044.1985614
CGTCTGA1183400.044.18300616
CGGAAGA1209000.044.1328624
GAGCACA1203900.044.1011629
GAAGAGC1209350.044.0229266
AAGAGCA1209350.043.9749767
ATCGGAA1212750.043.9488222
GGAAGAG1213450.043.931665
GAACTCC1184350.043.9128421
CACGGTA1156150.043.9041231
TCTGAAC1189200.043.87586618
ACTCCAG1195600.043.86703523
Produced by FastQC (version 0.11.2)