FastQCFastQC Report
Fri 31 Oct 2014
LW269_GTGGCC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW269_GTGGCC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19049026
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC489007325.670987062540622TruSeq Adapter, Index 20 (98% over 51bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC400000.20998448949568338TruSeq Adapter, Index 20 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5425000.044.6882321
TCGGAAG5489250.044.584773
CGGAAGA5489650.044.5333374
GCACACG5453950.044.4456111
ATCGGAA5495600.044.4428332
GAAGAGC5490800.044.4123846
AGAGCAC5478300.044.4072848
CACACGT5454300.044.40521612
AGCACAC5466600.044.39338310
ACACGTC5458250.044.36154613
GGAAGAG5513800.044.307925
CACGTCT5462950.044.29791614
AAGAGCA5504600.044.268697
ACGTCTG5463750.044.26754415
GAGCACA5491000.044.259929
CGTCTGA5466600.044.2004316
CTGAACT5475900.043.97692519
GAACTCC5452900.043.96233721
TCTGAAC5482250.043.9534818
GTCTGAA5498900.043.90386217
Produced by FastQC (version 0.11.2)