FastQCFastQC Report
Fri 31 Oct 2014
LW268_GTGAAA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW268_GTGAAA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3518713
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG56860316.159402599757357TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATTTCGTATG146230.41557808209990416TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTATG77780.2210467293013099TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAAGATCTCGTATG56980.1619342071945055TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTGAAACGATCTCGTATG45760.13004754863496965TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTGAAACGATCTCGTATG37900.1077098359542253TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT698600.044.4851912
ACACGTC693900.044.48496613
GCACACG699800.044.4603511
CACGTCT695050.044.353114
ACGTCTG695400.044.33140615
CGTCTGA695900.044.28338616
AGCACAC703850.044.26206210
AGAGCAC709650.044.17938
GAGCACA710400.044.0629849
GAACTCC692950.044.03796421
CTGAACT695450.044.01117319
TCTGAAC696200.043.9993118
AGTCACG698100.043.98778528
TCGGAAG715000.043.9752163
TGAACTC698800.043.96241820
GAAGAGC716550.043.9359866
GTCACGT692100.043.93162529
TCCAGTC695700.043.9247225
GATCGGA707650.043.916041
ACTCCAG693500.043.91481823
Produced by FastQC (version 0.11.2)