FastQCFastQC Report
Fri 31 Oct 2014
LW267_GTCCGC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW267_GTCCGC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3236355
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG44902113.874281406088022TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTCCGCACATCTCGTATG39620.12242167500166083TruSeq Adapter, Index 18 (97% over 40bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG38480.11889919369166857TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTCCGCACATCTCGTATG38400.1186520020207919TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC549650.044.13099313
GCACACG550750.044.12864311
GATCGGA551550.044.10681
TCGGAAG558000.044.0781023
CACACGT551300.044.0642112
AGCACAC553400.043.9532410
CGTCTGA551300.043.94857816
TCGTATG533500.043.85612545
CGGAAGA560400.043.837144
CACGTCT552400.043.82440614
ACGTCTG553550.043.80652215
AGAGCAC557600.043.7956778
AGTCACG549100.043.76544628
GAGCACA558100.043.7201589
GAAGAGC561200.043.7138376
ACGTCCG533100.043.7095232
CTGAACT549800.043.707719
CGTCCGC528800.043.69905533
GTCCGCA528450.043.68116434
CACGTCC533750.043.66050731
Produced by FastQC (version 0.11.2)