FastQCFastQC Report
Fri 31 Oct 2014
LW266_GTAGAG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW266_GTAGAG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2893128
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC94400.3262904372015341TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG17150.037.7826611
CGTAGAG11900.036.86755433
ACACGTC17650.036.839813
CACGTCT17650.036.83916514
AGTCACG15050.036.18037428
CACACGT18300.035.5312912
ACGTCTG18950.034.43066815
CAGTCAC16950.034.2487127
AGCACAC19550.033.48963510
CGTCTGA19450.033.42988616
GTCACGT15150.032.67301629
ACTCCAG19250.032.61126723
GTATGCC16500.032.3235245
CCAGTCA18450.032.31794426
TCACGTA14450.032.23047330
CACGTAG14600.032.20698531
GATCTCG15250.032.01891339
GAACTCC20100.032.01578521
TCCAGTC19050.031.53628325
GATCGGA20450.031.4759981
Produced by FastQC (version 0.11.2)