FastQCFastQC Report
Fri 31 Oct 2014
LW264_AGTTCC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW264_AGTTCC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3641694
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG148800.40860105214770925TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22500.036.09876611
CACACGT22600.036.03859312
ACACGTC22900.035.76296613
ACGTCTG23250.035.22411715
CGTATCT20450.034.9894639
CACGTCT23550.034.8704614
CCGTATC21050.034.4192438
CGTCTGA24200.033.74838316
CTCGTAT22000.033.04203444
GAACTCC25200.032.9462121
TCCGTAT22000.032.72570437
ACTCCAG25150.032.65340823
GTTCCGT22550.032.42593835
CAGTCAC23600.032.31925627
TCGTATG22650.031.99444845
GTCACAG23200.031.51745429
AGTTCCG23200.031.22652434
AGCACAC26650.031.1527810
GATCGGA27100.030.7252561
TTCCGTA23850.030.56415436
Produced by FastQC (version 0.11.2)