FastQCFastQC Report
Fri 31 Oct 2014
LW263_AGTCAA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW263_AGTCAA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493955
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC126610.5076675401119908TruSeq Adapter, Index 13 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG17700.038.77066811
ACGTCTG18250.037.47744815
CACGTCT18450.037.43702314
ACACGTC18700.036.8176913
CGTCTGA18600.036.65126416
CACAGTC17100.036.3131231
CCAGTCA18200.036.2205926
CAGTCAC18000.035.99807727
GTCACAG17750.035.870629
CACACGT19100.035.8110412
CGTATGC18100.035.56270644
GATCGGA19650.035.2773131
CTCGTAT18400.034.8605642
AGCACAC19750.034.85959210
GTATGCC18600.034.6067245
ACTCCAG19800.033.86182823
GAACTCC19950.033.8327821
ACAGTCA18350.033.59425732
TCCAGTC19750.033.37796425
TCGTATG19300.033.35155543
Produced by FastQC (version 0.11.2)