FastQCFastQC Report
Fri 31 Oct 2014
LW262_CTTGTA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW262_CTTGTA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3246383
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC67480.20786210376286468TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG11350.032.90620411
ACGTCTG11700.032.1131415
CACACGT11950.031.25401112
GTCACCT9700.030.85026429
ACACGTC12400.030.84557213
AGTCACC10200.030.22173928
CACGTCT12800.029.70495614
CGTATGC10700.029.23233444
CGTCTGA13150.028.91433116
CCAGTCA12300.027.989326
ACTCCAG13350.027.3048323
GTATGCC11600.026.96430645
GATCGGA14100.026.8157251
GAACTCC13700.026.77150221
TCCAGTC13200.026.76279325
TCGTATG12150.026.29932243
AGCACAC14500.025.75761810
CAGTCAC12600.025.71565827
CACCTTG11700.025.57553131
CTCGTAT12250.025.53354842
Produced by FastQC (version 0.11.2)