FastQCFastQC Report
Fri 31 Oct 2014
LW261_GGCTAC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW261_GGCTAC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5368560
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC721031.3430603364775657TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA129600.042.93222432
GTCACGG140050.042.73294429
CGGCTAC132300.042.5832533
TCACGGC136550.042.4112530
AGTCACG138950.042.40775328
CACGGCT135950.042.36673731
GGCTACA126550.042.2416134
GTATGCC128600.042.07024845
CGTATGC129850.041.7172644
CTCGTAT133000.041.57333442
ACGTCTG164650.041.5268715
GCACACG164850.041.31271411
CACGTCT165650.041.16752214
ACACGTC164800.041.16142313
CAGTCAC141000.041.12252827
ACTCCAG148450.041.08901623
CCAGTCA140100.041.0807926
GCTACAT130100.041.0025135
TCGTATG138850.040.98909843
TCCAGTC148850.040.84255625
Produced by FastQC (version 0.11.2)