FastQCFastQC Report
Fri 31 Oct 2014
LW259_GATCAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW259_GATCAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6657936
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC2077313.120051018814239TruSeq Adapter, Index 9 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGCC107640.16167172529144166TruSeq Adapter, Index 9 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTCTGCC104360.15674527361032006TruSeq Adapter, Index 9 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCAGATCTCGTATGCC90890.13651377844425058TruSeq Adapter, Index 9 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCGATCTCGTATGCC73790.1108301431554764TruSeq Adapter, Index 9 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC339650.043.62612545
GCACACG392800.043.42231411
ACGTCTG396850.043.3870415
ACACGTC392150.043.33331713
AGTCACG362150.043.29684428
CACACGT392900.043.245212
GTCACGA352000.043.1256629
CACGTCT397850.043.10833414
CGTATGC350050.043.0114944
CGTCTGA390850.042.9766516
CCAGTCA361300.042.88709326
TCGTATG361950.042.8524243
CAGTCAC362600.042.8152827
ACTCCAG372350.042.7745123
CTCGTAT355650.042.7692642
CACGATC344800.042.70156531
GATCTCG358800.042.69227639
GAACTCC374850.042.6626521
AGCACAC393450.042.5674910
CGATCAG345650.042.55749533