FastQCFastQC Report
Fri 31 Oct 2014
LW258_ACTTGA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW258_ACTTGA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8813316
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1834242.081214380603169TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC213650.042.06002829
ACGTCTG221900.041.6413115
CAGTCAC218100.041.62764427
GTATGCC214950.041.53317645
CACGTCT223400.041.51278314
CGTCTGA222400.041.48699616
ACACGTC224150.041.44437813
GCACACG224600.041.4414811
CCAGTCA221050.041.30622526
GAACTCC222600.041.30162421
ACTCCAG222250.041.24518223
AGTCACA219550.041.06482728
TCCAGTC223250.040.96972725
CACACGT227200.040.9375312
CGTATGC219750.040.70789344
AGCACAC230050.040.5279410
CTCGTAT222400.040.27343442
TCACACT221250.040.2685930
AATCTCG221100.040.1996339
CTCCAGT227550.040.19552224
Produced by FastQC (version 0.11.2)