FastQCFastQC Report
Fri 31 Oct 2014
LW257_CAGATC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW257_CAGATC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6044641
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC4418817.310293531079844TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC507350.043.78110545
AGTCACC505000.043.43288828
CTCGTAT512400.043.35840242
CGTATGC512400.043.34961744
TCGTATG514650.043.2650443
CAGTCAC515650.043.16526827
CCAGTCA515000.043.09741226
GTCACCA508350.043.0154629
ACTCCAG518350.042.96177723
ACCAGAT508500.042.95268232
ACGTCTG525200.042.9277215
TCTCGTA517550.042.91940741
TCCAGTC517900.042.90352225
CACACGT527050.042.8805512
ACACGTC527000.042.8760813
CACGTCT526250.042.85453814
CCAGATC504100.042.8541533
GCACACG527750.042.832211
CGTCTGA526500.042.79608516
TCACCAG511750.042.73705330