FastQCFastQC Report
Fri 31 Oct 2014
LW256_GCCAAT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW256_GCCAAT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4767624
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC4121658.645081910821826TruSeq Adapter, Index 6 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTCTGCC223320.46840942154834353TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACTATCTCGTATGCC198950.417293813438308TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATCTCTCGTATGCC173010.36288516040694485TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCCATATCTCGTATGCC161700.3391626520883358TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCGCCAATATCTCGTATGCC135900.285047646374798TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGAC87530.18359249806612266TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACGCCAATATCTCGTATGCC80930.1697491245114967TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTACAGTCACGCCAATATCTCGTATGCC74330.15590575095687076TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTAACGCCAATATCTCGTATGCC73280.15370339607318026TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATGCC62360.13079890528279914TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCA60550.12700246495948506TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGCGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC52730.11060016477809492TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACGCCAATATCTCGTATGCC51390.10778954045033752TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAAATATCTCGTATGCC51050.10707639696419012TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAATATCTCGTATGCC49890.10464331918792255TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTATGCC47800.10025958422895766TruSeq Adapter, Index 6 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT819800.044.25945312
CACGTCT822750.044.22015414
ACACGTC819700.044.18981613
AGCACAC811050.044.1553210
TCGGAAG833900.044.138343
GCACACG823500.044.12889511
ACGTCTG828750.044.09546715
AGAGCAC814600.044.0839588
CGTCTGA818450.044.02364716
GTATGCC705900.043.96653745
ATCGGAA833650.043.962672
GATCGGA844000.043.952011
GAGCACA822600.043.9205369
AAGAGCA813600.043.905857
CGGAAGA824350.043.8809664
TCTGAAC804500.043.84411618
GTCTGAA816250.043.83313817
GAAGAGC820850.043.819576
GGAAGAG829550.043.7767645
CTGAACT806500.043.76000219
Produced by FastQC (version 0.11.2)