FastQCFastQC Report
Fri 31 Oct 2014
LW255_ACAGTG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW255_ACAGTG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4118030
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC224740.5457463884430177TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC43950.037.43196544
ACGTCTG50200.037.42269515
TCGTATG45350.037.26846343
GTATGCC43850.037.00409745
GCACACG49750.036.85672811
CTCGTAT43800.036.84036342
CACACGT50300.036.5879112
ACACGTC50950.036.29776813
GTCACAC44500.036.09948729
CGTCTGA50100.036.06036416
CACGTCT51850.036.05824314
CACAGTG45350.035.67049833
GAACTCC48450.035.61844621
CCAGTCA46750.035.5665326
GATCTCG45450.035.50163339
TCCAGTC47900.035.46419525
CAGTCAC46750.035.42257727
ACTCCAG49100.035.1931723
AGTGATC44450.035.17795636
GATCGGA55000.035.022551
Produced by FastQC (version 0.11.2)