FastQCFastQC Report
Fri 31 Oct 2014
LW254_TGACCA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW254_TGACCA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5539840
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC595171.074345107439926TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC106500.040.7834645
CGTATGC108700.040.3928144
CAGTCAC115300.040.0809227
ACTGACC108650.040.06913432
CTCGTAT111400.039.99857742
CTGACCA105700.039.95287333
TCGTATG113600.039.89822443
ACGTCTG130300.039.71386315
CGTCTGA127450.039.6310216
GTCACTG122950.039.56305729
ACACGTC128850.039.51471313
CACGTCT130600.039.4331414
GCACACG129800.039.38186611
CCAGTCA117600.039.37355426
CACACGT129450.039.26239812
ACTCCAG121400.039.23455423
TCCAGTC121150.039.2040925
TCTCGTA115100.038.9474141
GAACTCC124100.038.92448821
CACTGAC114600.038.8136731
Produced by FastQC (version 0.11.2)