FastQCFastQC Report
Fri 31 Oct 2014
LW253_GGTAGC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW253_GGTAGC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3020753
Sequences flagged as poor quality0
Sequence length51
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC2352257.78696570027407TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTCTGCC161190.5336086730692645TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCGCATCTCGTATGCC103590.34292774020252564TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCCTCTCGTATGCC100570.33293023295847096TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGAC68250.22593704284991192TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCGGTAGCATCTCGTATGCC58540.19379273975727243TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACGGTAGCATCTCGTATGCC53690.17773714037526406TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGAATCTCGTATGCC51760.17134800495108338TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATATCGTATGCC35670.11808314019716276TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCA35640.11798382721129466TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGCGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC35100.11619619346566899TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACGGTAGCATCTCGTATGCC34060.11275334328890842TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTACAGTCACGGTAGCATCTCGTATGCC33910.11225677835956796TruSeq Adapter, Index 11 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAT381050.043.92859335
TAGCATC372550.043.85589636
GGTAGCA381800.043.83640734
CACGGTA394600.043.77713431
AGTCACG421400.043.73872428
GTATGCC378250.043.72697445
ACGGTAG400850.043.6951332
CACACGT461950.043.65793612
CAGTCAC419400.043.59323527
ACACGTC464050.043.56702813
CTCGTAT390300.043.5641142
CACGTCT467250.043.5527514
CGGTAGC399300.043.5210433
ACTCCAG432850.043.51215723
CCAGTCA417100.043.50996426
TCCAGTC432200.043.50992225
CTCCAGT437150.043.50619524
TCACGGT418450.043.48644330
TCGTATG397700.043.48423843
AACTCCA422400.043.48070522
Produced by FastQC (version 0.11.2)