FastQCFastQC Report
Fri 31 Oct 2014
LW251_CGTACG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW251_CGTACG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9178580
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC96759910.541924785751172TruSeq Adapter, Index 22 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGCTCTCGTATGCC594820.6480523131028983TruSeq Adapter, Index 22 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTCTGCC582860.6350219750767547TruSeq Adapter, Index 22 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACGATCTCGTATGCC233080.253939062469358TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACCGTACGATCTCGTATGCC224630.2447328453856697TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGAC210810.2296760501079688TruSeq Adapter, Index 22 (97% over 49bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACCGTACGATCTCGTATGCC196990.21461925483026786TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGATCTCGTATGCC195670.21318112387754967TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCCGTACGATCTCGTATGCC189680.20665505993301797TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCA155420.16932902475110528TruSeq Adapter, Index 22 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATATCGTATGCC149580.16296638477847336TruSeq Adapter, Index 22 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAAGATCTCGTATGCC131950.14375862061451772TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGACCTCCAGTCACCGTACGATCTCGTATGCC128100.13956407200242305TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTAGTATGCC123800.13487925147462898TruSeq Adapter, Index 22 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTACAGTCACCGTACGATCTCGTATGCC119440.13012906135807498TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGCGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC104290.11362324019619592TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCAAGTCACCGTACGATCTCGTATGCC102030.11116098568623904TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGCAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC100420.10940690172118127TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACGTACGATCTCGTATGCC99590.10850262241000241TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTAACCGTACGATCTCGTATGCC98300.1070971762516642TruSeq Adapter, Index 22 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA1524350.043.6648334
GTACGAT1576850.043.6464235
CCGTACG1610900.043.6405433
ACCGTAC1585150.043.55603432
TACGATC1533350.043.51612536
CACCGTA1598000.043.4789331
AGTCACC1614050.043.47546828
CTCCAGT1721900.043.41540524
CAGTCAC1658550.043.39201727
CCAGTCA1657000.043.35473626
AACTCCA1669500.043.3293122
ACTCCAG1709800.043.3239323
TCCAGTC1704400.043.3133425
GAACTCC1725450.043.29561621
TCTGAAC1801950.043.25436418
CACGTCT1870100.043.24690214
CACACGT1861250.043.23663312
TCTCGTA1589050.043.230641
GTATGCC1547350.043.22239745
TGAACTC1771300.043.22133620
Produced by FastQC (version 0.11.2)