FastQCFastQC Report
Fri 31 Oct 2014
LW250_GTTTCG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW250_GTTTCG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3653806
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCC888742.4323677830733215TruSeq Adapter, Index 21 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGCTCTCGTATGCC54840.15009007046351122TruSeq Adapter, Index 21 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTCTGCC48900.13383304970214618TruSeq Adapter, Index 21 (97% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC135850.042.5930245
AGTCACG144650.042.3240528
CTCGTAT140450.042.27047742
CGTATGC138250.042.1791944
GCACACG159350.042.1167211
TCGTATG143450.042.0924543
GTCACGT147550.042.06992329
ACACGTC158650.042.06146613
CACACGT159400.042.03293612
ACGTCTG160550.041.9981115
CAGTCAC145450.041.84375827
CACGTCT160500.041.71682414
CGTCTGA159400.041.68007316
CCAGTCA145350.041.60881826
ACTCCAG150900.041.52475723
TCTCGTA143050.041.5016241
GAACTCC152600.041.45968621
AGCACAC159900.041.42310710
CGTTTCG149950.041.3065633
TCCAGTC150950.041.16818225
Produced by FastQC (version 0.11.2)