FastQCFastQC Report
Fri 31 Oct 2014
LW249_GTGGCC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW249_GTGGCC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3582001
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC1859855.192209605748296TruSeq Adapter, Index 20 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTCTGCC118590.331071934374111TruSeq Adapter, Index 20 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCCTCTCGTATGCC86910.24262974800956225TruSeq Adapter, Index 20 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGACATCTCGTATGCC51280.1431602057062519TruSeq Adapter, Index 20 (97% over 39bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACGTGGCCATCTCGTATGCC50720.14159683372506038TruSeq Adapter, Index 20 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACGTGGCCATCTCGTATGCC48930.13659962685660892TruSeq Adapter, Index 20 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGAC43610.1217475930352895TruSeq Adapter, Index 20 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCGTGGCCATCTCGTATGCC41680.11635954317154017TruSeq Adapter, Index 20 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCAATCTCGTATGCC38570.10767724520456583TruSeq Adapter, Index 20 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC299750.043.42430545
AGTCACG330600.043.3111928
GTGGCCA304700.043.23297534
CTCGTAT311300.043.21439442
ACGTCTG365500.043.20644815
CAGTCAC327550.043.15122227
GTCACGT337600.043.13958729
TGGCCAT306000.043.13018435
CACACGT356000.043.1086912
TCACGTG335550.043.04776830
CGTATGC306450.043.0403644
GCACACG357350.043.03397811
ACACGTC358300.043.03230713
CACGTCT363300.043.0283914
CGTCTGA360550.042.96969216
ACTCCAG336050.042.94903623
GCCATCT305250.042.9369637
CGTGGCC320150.042.93282333
CCAGTCA325100.042.92848226
TCCAGTC334900.042.9151325
Produced by FastQC (version 0.11.2)