FastQCFastQC Report
Fri 31 Oct 2014
LW247_GTCCGC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW247_GTCCGC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6150585
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG4099496.665203391222136TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTCTG263350.42817065368578766TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACCTCTCGTATG191540.31141753182827325TruSeq Adapter, Index 18 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCCCATCTCGTATG160330.2606743911351522TruSeq Adapter, Index 18 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGCACATCTCGTATG98820.1606676438094913TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACGTCCGCACATCTCGTATG96380.15670054149320756TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCGTCCGCACATCTCGTATG87200.14177513195899252TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGAACATCTCGTATG76550.12445970586537702TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACGTCCGCACATCTCGTATG71530.11629788060810477TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATATCGTATG71070.11554998426978896TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTACAGTCACGTCCGCACATCTCGTATG67590.10989198588426954TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACGCACATCTCGTATG65930.10719305561991259TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG743250.043.98253645
ACGTCCG744950.043.7753432
GTCCGCA714650.043.6902234
AGTCACG786950.043.6894528
CGCACAT685600.043.6697237
CGTCCGC741100.043.66272733
TCCGCAC699550.043.62021335
GTCACGT796550.043.61731329
CACGTCC739050.043.5524731
TCACGTC773550.043.53261630
CCGCACA670000.043.49605636
CTCGTAT735750.043.461244
CAGTCAC781300.043.45318227
ACTCCAG784750.043.39298223
CCAGTCA771550.043.31815326
GCACATC702600.043.28809738
TCCAGTC793250.043.2853925
GAACTCC797950.043.2584721
TCTCGTA744400.043.22954643
CTCCAGT796450.043.22164524
Produced by FastQC (version 0.11.2)