FastQCFastQC Report
Fri 31 Oct 2014
LW246_GTAGAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW246_GTAGAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4408512
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC58210.13204001713049662TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG14950.028.74554428
CGTATGC15000.028.05641444
CGTAGAG13850.027.77857433
GTATGCC15000.027.15620845
ACACGTC19100.026.73906313
CACGTCT19550.026.58391614
GCACACG19350.026.51017211
ACGTCTG20200.026.06262815
CACACGT19450.026.02684812
GTCACGT16750.025.7899229
CGTCTGA20200.025.7284916
TCGTATG16900.025.70026843
TCACGTA16400.025.3799930
CACGTAG17200.024.19952431
CAGTCAC18700.023.70298227
ACGTAGA16650.023.64761732
GAACTCC21050.023.51441421
CTCGTAT18050.023.31452242
CCAGTCA19700.022.84190226
ACTCCAG21150.022.7654823
Produced by FastQC (version 0.11.2)