FastQCFastQC Report
Fri 31 Oct 2014
LW245_CCGTCC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW245_CCGTCC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4834751
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG671291.3884686098622245TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTATG57270.11845491112158621TruSeq Adapter, Index 16 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTCTG50230.10389366484437358TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG121350.043.32888435
CCGTCCC118750.042.98921234
GTCCCGA112700.042.8414736
TCACCCG126600.042.7769630
GTCACCC127650.042.70710429
ACGTCTG151150.042.70521515
CCCGTCC122550.042.5750333
GCACACG149150.042.55380211
ACCCGTC125600.042.50848832
CACGTCT150500.042.4710814
AGTCACC128600.042.40955428
CGTCTGA151650.042.38638716
CACCCGT127550.042.33487731
CCCGATC111800.042.0860538
GAACTCC144100.041.8128421
CCGATCT116600.041.53288739
CAGTCAC135800.041.4036427
ACTCCAG141250.041.36671423
ACACGTC153750.041.32455413
AGCACAC152200.041.19887510
Produced by FastQC (version 0.11.2)