FastQCFastQC Report
Fri 31 Oct 2014
AH8038D-141013_GTGAAA_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH8038D-141013_GTGAAA_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19183302
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG741380.38647152612204094TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG96550.036.91220528
GATCGGA101250.036.7648851
GCACACG99650.036.5311811
ACACGTC100450.035.9491613
GTCACGT100050.035.39576329
ACGTCTG102800.035.06170715
TCACGTG102300.034.68306730
CACACGT105550.034.6171612
CACGTCT105150.034.40649814
CACGTGA103900.034.34376531
CTCGTAT102050.034.26827244
CGTCTGA106000.033.9819316
ATCTCGT108550.032.3199442
TCGTATG110250.032.2299845
TCGGAAG115950.032.191363
TCTCGTA109300.032.05698843
ATCGGAA119950.031.1375182
GATCTCG113650.031.02735541
CGATCTC112550.030.77068740
AACGATC113750.030.44321438
Produced by FastQC (version 0.11.2)